Daffy Duck first appeared in the 1937 short Porky's Duck Hunt, directed by Tex Avery and animated by Bob Clampett.

Multiple sources confirm this origin date and creative team. The Chuck Jones website states that Daffy first appeared in Porky's Duck Hunt, released on April 17, 1937, and that the cartoon was directed by Tex Avery with Bob Clampett as animator. Britannica corroborates this, noting Daffy first appeared in director Tex Avery's and animator Bob Clampett's short Porky's Duck Hunt (1937). The Simple Wikipedia entry for Daffy Duck also lists the first appearance as Porky's Duck Hunt on April 17, 1937, with creators Tex Avery and Bob Clampett.